Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application.How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown.Here, using intra-molecular single-molecule fluorescence resonance energy transfer measurements, we revealed that Cas9 in apo, sgRN
Teklu Gebrehiwot Gebremichael,1 Hansa Haftu,2 Teklebrhan Aregawi Gereziher11Clinical Pharmacy Department, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia; 2Pediatrics and Child Health Department, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, EthiopiaBackground: Iron folate supplem
Background: There is increasing interest in investigating genetic risk models in empirical studies, but such studies are premature when the expected predictive ability of the risk model is low.We assessed how accurately the predictive ability of genetic risk models can be estimated in simulated data that are created based on the odds ratios (ORs) a
Laurel wilt (LW) is a vascular disease caused by a fungal pathogen transmitted to avocado trees by several ambrosia beetle species and through root grafts among adjacent avocado trees.A critical part of preventing and controlling plant diseases ds durga hand soap is determining the causal agent so that the appropriate management practices can be im